An integrated analysis of plant and bacterial gene expression in symbiotic root nodules using laser-capture microdissection coupled to RNA sequencing

Rhizobium-induced root nodules are specialized organs for symbiotic nitrogen fixation. Indeterminate-type nodules are formed from an apical meristem and exhibit a spatial zonation which corresponds to successive developmental stages. To get a dynamic and integrated view of plant and bacterial gene expression associated with nodule development, we used a sensitive and comprehensive approach based upon oriented high-depth RNA sequencing coupled to laser microdissection of nodule regions.

See also

B.Roux, N.Rodde, M-F.Jardinaud, T.Timmers, L.Sauviac, L.Cottret, S.Carrère, E.Sallet, E.Courcelle, S.Moreau, F.Debellé, D.Capela, F.Carvalho-Niebel, J.Gouzy, C.Bruand, P.Gamas

Modification date: 07 June 2023 | Publication date: 19 June 2014 | By: G. Esteve