Accueil
The LabEx
Scientific attractiveness
Visiting scientists
Visiting Scientists workshop by Federico Ariel

Visiting Scientists workshop by Federico Ariel

A TULIP Workshop organized with Federico Ariel – from The Instituto de Agrobiotecnología del Litoral in Santa Fe, Argentina – on Thursday December 15th from 14H to 17H. Meet him in the FR-40 room!

This Workshop is a part of his staying at LIPM from December 1st to 18th as a "Visiting Scientist" supported by labex TULIP. Federico will introduce us to a wide range of chromatin biochemical techniques, including ChIP, RIP, ChIRP and 3C. Finally, he will integrate the use of these approaches in the context of his studies on dynamic chromatin 3D conformation and its impact on gene activity in Arabidopsis thaliana.

Figure3BFedericoAriel

(B) APOLO transcription by Pol II and Pol V controls chromatin loop dynamics to fine-tune promoter activity of the neighboring gene PID. (i) Auxin activates APOLO DNA demethylation and ‘opens’ the loop encompassing the PID promoter region. (ii) Pol II divergent transcription leads to increased accumulation of PID and APOLO RNAs. Pol II APOLO transcripts gradually recruit PRC1 components (blue balls) to mediate loop ‘closing’. Pol V transcripts are recognized by AGO4 loaded with Pol IV-derived 24 nt siRNAs and trigger DNA methylation (‘m’). (iii) Pol II APOLO- mediated loop is finally re-formed and stabilized by Pol IV/Vdependent DNA methylation, downregulating levels of PID transcripts. PID exons are represented in green, the APOLO locus in red, and RNAs are red arrows. Transcription start-sites are indicated by black arrows. Model based on [95].

Modification date : 07 June 2023 | Publication date : 15 November 2016 | Redactor : TULIP Communication